Figure 2.

Sequence similarity network of the AdoMet radical enzyme subfamily, 7‐carboxy‐7‐deazaguanine synthases (QueE). The protein sequence similarity network38 for the QueE AdoMet radical subfamily was obtained from the Structure Function Linkage Database (http://sfld.rbvi.ucsf.edu/django) and visualized in Cytoscape.39 Each node represents sequences that share 50% identity or higher and node connections are filtered at a Blast Probability of 10⁻²⁵. Nodes are colored based on increasing sequence length; White nodes denote the shortest sequences (149 amino acids) and the orange node denotes the longest sequence (509 amino acids). B. multivorans, B. subtilis and E. coli QueE sequences are shown as diamonds and sequences used in the sequence alignment (Fig. S2) are shown as hexagons and designated with an asterisk (*). QueE catalyzes the AdoMet and magnesium dependent rearrangement of 6‐carboxy‐5,6,7,8‐tetrahydropterin, CPH_4, to 7‐carboxy‐7‐deazaguanine, CDG.