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. 2018 Oct 31;18(12):7494–7501. doi: 10.1021/acs.nanolett.8b02916

Figure 2.

Figure 2

Spectral analysis of 70 μM αS aggregation by sPAINT. (a) Representative sPAINT hydrophobicity images of single ThT active αS aggregates at different time-points (1h, 3h, 9h, 24h, and 48 h) with a hydrophobicity scale. Scale bar is 100 nm in 1, 3, 9, and upper 24 h, and 1 μm in bottom 24 and 48 h. (b) Total hydrophobicity populations of non- ThT active and ThT active αS species (1 h, N(non-ThT) = 551, N(ThT) = 119; 3 h, N(non-ThT) = 1,091, N(ThT) = 334; 9 h, N(non-ThT) = 1,143, N(ThT) = 401; 24 h, N(non-ThT) = 1,094, N(ThT) = 820; 48 h, N(non-ThT) = 502, N(ThT) = 538). (c) Hydrophobicity landscapes of individual aggregates at the different time-points with a density scale. (Lookup table: population density) (d) The number of αS species per μm2 as a function of time. These data correspond to the mean and standard deviation of five independent experiments. (e) PK digestion assays of a 2 day αS aggregation. The fraction of the number of αS species in the PK-exposed sample (1 h) was divided by the number of αS species in the initial sample (total number of αS species: N(non-ThT) = 90, N(ThT) = 185). These data correspond to the mean and standard deviation of three independent experiments.