Figure 5.

(a) Hydrophobicity heterogeneity (mean hydrophobicity versus hydrophobicity variability) of non-ThT active and ThT active species from all time points for the 70 μM αS aggregation. (Lookup table: population density). (b) Histogram of hydrophobicity variability at 610 nm through the center of (A) and (B) and at 633 nm through the center of (C) using the data in Figure 5a. (c) Mean and standard deviation (error bars) of the hydrophobicity variability of oligomeric species at early stages (non-ThT active and ThT active species) and ThT active mature fibrillar species at later-stages with a mean hydrophobicity over 630 nm and ellipticity less than 0.2. The measured width of oligomeric species is larger than that of mature fibrillar species (p < 0.0001 by t test).