Table 1. Comparison of Different Single-Molecule Plasmon Resonance Sensing Methods.
| method | time resolution (ms) | waiting time | max no. of events | specificity | ref |
|---|---|---|---|---|---|
| photothermal microscopy | 100 | tens to hundreds of seconds (100 nM protein) | <10 (streptavidin) | yes | (10) |
| dark-field spectroscopy | 24 | 50 s (average, 1.25 μg/mL fibronectin) | ∼10–20 (fibronectin) | yes | (9) |
| single-nanorod scattering | 6 | ∼1–100 s (25–2.5 nM antibody) | ∼10b (antibody) | yes | (11) |
| double-nanohole plasmonic trapping | <1 | seconds to hoursa | not determined (no binding site consumed) | no | (5) and (6) |
| plasmonic nanopores | <200 μs | ∼100 ms (1 μg/mL dsDNA) | >500 (no binding site consumed) | no | this work |
a
Waiting times in these devices vary widely depending on slight differences in sample preparation and poorly understood surface repulsion.6
b
On each nanorod.