Table 3.
Primers aimed at the replicase gene used in monitoring studies of coronaviruses in wild birds
| Method | Primer | Genome position* | Amplicon (bp) | Reference |
|---|---|---|---|---|
| 1 | 2Bp | 13960–13982 | ||
| 4Bm | 14189–14210 | 251 | (37) | |
| Pol1 | 14188–14210 | |||
| 2 | Pol2 | 14604–14627 | 440 | (51) |
| Pol1 | 14188–14207 | |||
| Pol2 | 14770–14789 | 600 | ||
| 3 | Pol3 | 14188–14210 | (11) | |
| Pol4 | 14605–14627 | 440 | ||
| 11-FW | 14619–14642 | |||
| 4 | 13-RW | 14773–14797 | 179 | (33) |
*
primer positions refer to the sequences of the gammacoronavirus representative, IBV M41 strain (GenBank accession no.: DQ834384)