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. 2018 Dec 17;23:57. doi: 10.1186/s11658-018-0123-z

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Cellular internalization of HUVEC-derived EVs into HUVECs. HUVECs were incubated for 24 h with EVs labelled with PKH26 (red). The carryover of PKH26 was observed when cells were incubated with PKH26 without EVs (negative control). a, b – Transmitted light. c, e – PKH26 staining. d, f – DAPI staining. g, i – Merged 2D view. h, j – Merged 3D view. k, l – 3D horizontal view