Fig. 2.

Up-regulated expression of TRIP13 is associated with the progression of HCC and is in HCC cell lines. a HCC patients with different stages were detected the mRNA expression of TRIP13 in its tumor tissue and adjacent normal tissue. b Six randomly selected HCC patients was detected the protein expression of TRIP13 in its tumor tissue and adjacent normal tissue. c Related TRIP13 expressions was showed as bar diagrams. GAPDH served as an internal control. Data were expressed as mean ± SD from three independent experiments (*compared with adjacent tissues, *P < 0.05, **P < 0.01). Normal hepatocytes LO2 cells and six HCC cell lines, including SNU-886, HepG2, BEL7405, HCCC9810, SMMC-7721 and MHCC97H were used to detect the mRNA (d) and protein expression (e) of TRIP13. GAPDH served as an internal control. Data were expressed as mean ± SD from three independent experiments (*compared with LO2 cells, *P < 0.05, **P < 0.01)