. 2018 Dec 17;11:643. doi: 10.1186/s13071-018-3192-y

Table 1.

Primers used in the detection of various tick-borne pathogens and control genes

Organism	Target gene	Primer name	Sequence (5'-3')	Annealing T (°C)	Product size (bp)	Reference
Anaplasma/Ehrlichia spp.	groEL	gro607F	GAAGATGCWGTWGGWTGTACKGC	57	664	[35]
		gro1294R	AGMGCTTCWCCTTCWACRTCYTC	57	664
		gro677F	ATTACTCAGAGTGCTTCTCARTG	57	315
		gro1121R	TGCATACCRTCAGTYTTTTCAAC	57	315
Anaplasma platys	groESL	PLA-HS475F	AAGGCGAAAGAAGCAGTCTTA	63	513	[36]
Anaplasma platys	groESL	PLAT-HS1198R	CATAGTCTGAAGTGGAGGAC	63	513	[36]
Ehrlichia canis	gltA	EcanisFw	TTATCTGTTTGTGTTATATAAGC	53	1372	[37]
Ehrlichia canis	gltA	EcanisRev	CAGTACCTATGCATATCAATCC	53	1372	[37]
Rickettsia spp.	gltA	CS2d	ATGACCAATGAAAATAATAAT	52	1250	[38]
		CSEndr	CTTATACTCTCTATGTACA	52	1250
		RpCs877p	GGGGGCCTGCTCACGGCGG	52	341
		RpCs1258n	ATTGCAAAAAGTACAGTGAAC	52	341
Babesia/Hepatozoon	18S rRNA	piro18S F1	GGTGAAACTGCGAATGGCTC	55	1500	This study
		piro18S R1	AAGTGATAAGGTTCACAAAACTT	55
		piro18S F2	TGGCTCATTACAACAGTTATA	53
		piro18S R2	CGGTCCGAATAATTCACC	53
Babesia canis	18S rRNA	BcanisF	GTTTATTAGTTTGAAACCCGC	59	456	[39]
Babesia canis	18S rRNA	BcanisR	GAACTCGAAAAAGCCAAACGA	59	456	[39]
Hepatozoon canis	18S rRNA	HepF	ATACATGAGCAAAATCTCAAC	50	666	[23]
Hepatozoon canis	18S rRNA	HepR	CTTATTATTCCATGCTGCAG	50	666	[23]
Tick control	mt-rrs	mt-rrsF	CTGCTCAATGATTTTTTAAATTGCTGTGG	56	460	[40]
Tick control	mt-rrs	mt-rrsR	CCGGTCTGAACTCAGATCAAGTA	56	460	[40]
Blood control	actin	Actin-F	CGCACCACCGGCATCGTGAT	65	227	[41]
Blood control	actin	Actin-R	TCCAGGGCCACGTAGCAGAG	65	227	[41]

Abbreviation: T temperature