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. 2018 Dec 17;9:350. doi: 10.1186/s13287-018-1088-6

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Efficient endothelial induction from hADSCs by ETV2 transduction. a Schematic of the induction protocol. b Induced ETV2-hADSCs exhibited cobblestone appearance during EC induction. c Representative flow cytometric analysis of KDR+ cells in ETV2-hADSCs that treated with SB431542 (SB) at indicated time points. Cells cultured in EIM were treated as control. d Expression of endothelial-specific markers KDR, CD34, and NRP1 were measured by immunofluorescence staining 10 days post induction. Scale bar = 50 μm. e mRNA levels of endothelial-specific genes were determined by quantitative RT-PCR in ETV2-hADSCs at the indicated time points. Data are normalized to β-actin. f Endothelial-specific markers KDR, NRP1, and CD34 in ETV2-hADSCs were measured by flow cytometry during induction. *P < 0.05, **P < 0.01