Fig. 7.

Efficient endothelial induction from hUMSCs by ETV2 transduction. a A schematic of the optimal induction protocol. b Induced ETV2-hUMSCs exhibited cobblestone appearance during EC induction. c Quantitative and representative flow cytometric analysis of KDR in induced ETV2-hUMSCs treated with SB at indicated time points; cells cultured in basal medium were treated as control. d Phenotypic analysis of KDR+ cells after fluorescence-activated cell sorting. e, f Immunofluorescence showed expression of KDR, CD34, and NRP1 in induced ETV2-hUMSCs on 10 days post-induction. g mRNA levels of endothelial-specific genes were determined by quantitative RT-PCR in induced ETV2-hUMSCs at the indicated experimental time points. Data are normalized to β-actin. h EiECs derived from induced ETV2-hUMSCs were able to form tubular structures on Matrigel-coated plates. i The number of branches were quantified (n = 5). h–k Immunofluorescence of endothelial markers CD31, vWF, and ac-LDL uptake were detected in mature EiECs; hUVECs were used as positive control. Scale bar = 50 μm. *P < 0.05, **P < 0.01