Fig. 7.

Expression of p₁₇₃₄₀:P5CS2 restores fertility of p5cs1/p5cs2 double mutant pollen. a Seeds produced by wildtype pistils fertilized with pollen from either a p5cs sesquimutant (leftmost panel), or a p5cs sesquimutant heterozygous (middle panel) or homozygous (rightmost panel) for the p₁₇₃₄₀:P5CS2 construct were germinated on sulfadiazine-containing plates to score for the transmission of the p5cs2–1 allele. b Percentages of sulfadiazine-resistant seedling among the progeny of wildtype pistils after cross-pollination as described in a Bars represent the mean ± SE of, at least, three independent experiments with plants of two independent transgenic lines. nd: not detected; *** indicates significant difference to p₁₇₃₄₀:P5CS2 het (p < 0.001 by student’s T-test). Two further lines produced very similar results. c PCR analysis of the sulfadiazine-resistant progeny. Individual resistant plantlets were analyzed by PCR for the simultaneous presence of both the T-DNA generating the p5cs2–1 mutation (upper panel) and the p₁₇₃₄₀:P5CS2 construct (bottom panel). The sizes of the PCR products matched the expected numbers of base pairs (indicated alongside)