Table 4.
Blood redox parameters5 | ||||||||
---|---|---|---|---|---|---|---|---|
Vit Cμmol/l | SOD U/g Hb | GPx U/g Hb | CAT U/g Hb | GSH + GSSG μmol/l | FRAP μmol/l | MDA μmol/l | LOOH μmol/l | |
Treatment1 | ||||||||
DLML | 5.66 | 306 | 147b | 794 | 0.83ab | 1108a | 0.82 | 35.1 |
MHAL | 4.95 | 385 | 143b | 996 | 0.72c | 588c | 1.00 | 30.3 |
DLMH | 5.32 | 265 | 173a | 726 | 0.80b | 854b | 0.81 | 33.5 |
MHAH | 4.90 | 377 | 107c | 1119 | 0.89a | 959ab | 0.80 | 23.9 |
Dosage2 (D) | ||||||||
Low | 5.31 | 345 | 145 | 895 | 0.77 | 848 | 0.91 | 32.7 |
High | 5.11 | 321 | 140 | 922 | 0.84 | 906 | 0.81 | 28.7 |
Source3 (S) | ||||||||
DLM | 5.49 | 286 | 160 | 760 | 0.81 | 981 | 0.82 | 34.3 |
MHA | 4.92 | 380 | 125 | 1057 | 0.80 | 773 | 0.90 | 27.1 |
p-value | ||||||||
Dosage | 0.423 | 0.130 | 0.299 | 0.558 | 0.012 | 0.302 | 0.062 | 0.035 |
Source | 0.027 | < 0.001 | < 0.001 | < 0.001 | 0.634 | 0.001 | 0.139 | < 0.001 |
D × S | 0.559 | 0.303 | < 0.001 | 0.066 | 0.001 | < 0.001 | 0.088 | 0.190 |
SEM4 | 0.128 | 11.63 | 4.885 | 35.59 | 0.017 | 43.09 | 0.029 | 1.154 |
1Diets fed to turkeys containing DL-methionine (DLML and DLMH) and the equivalent amount of DL-methionine hydroxyl analogue (MHAL and MHAH) at two levels: low (L) and high - (H)
2Low level - 0.55 and 0.45% and high level - 0.78, 0.65%, in weeks 1–4 and 5–8 of feeding
3Sources of methionine: DL-isomer (DLM) and DL- methionine hydroxy analogue (MHA)
4SEM, standard error of the mean
5Vit C: vitamin C, SOD: superoxide dismutase, GPx: glutathione peroxidase, CAT: catalase, GSH + GSSG: total glutathione, FRAP: ferric reducing ability of plasma, MDA: malondialdehyde, LOOH: lipid peroxides
a-bvalues differ significantly