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. 2018 Dec 17;28(1):176–190. doi: 10.1002/pro.3517

Figure 7.

Figure 7

Limited proteolysis, hydrogen peroxide formation and SAXS data for Irc15p in the presence and absence of NADH. (A) SDS‐PAGE from the limited proteolysis experiment illustrating the effect of NADH and oxygen on the stability of the protein. Each gel has the marker PageRuler™ prestained protein ladder in Lane 1, the remaining lanes display the samples incubated for 0–16 h. (B) Hydrogen peroxide formation in Irc15p over time (0, 1, and 16 h) and in the presence and absence of NADH. (C) SAXS data comparing the experimental radial density distribution (P(r)) of Irc15p incubated with NADH measured after 0 and 12 h compared with a control sample without NADH.