Table 3.

Activity and Thermal Stability of Irc15p at Various pH and in the Absence and Presence of NaCl. Melting Points are Given as the Average of Two Independent Measurements

Buffer composition	Activity [%]	Tm [°C]
50 mM HEPES, pH 6.0	51	70
50 mM HEPES, pH 7.0	100	69
50 mM HEPES, pH 8.0	86	62
50 mM Tris/HCl, pH 9.0	46	56
50 mM HEPES, pH 7.0, 150 mM NaCl	61	69

The reaction mixture for the activity assay contained also 10 nM DTT, 500 μM NADH, and 500 μM ferricyanide.