Figure 2.

ICAM-1 expression in mice.

Notes: After euthanasia, the lung cells were collected and stained with CD45-PE-labeled and CD31-APC-labeled antibodies for 30 minutes at 25°C. The mouse lung microvascular endothelial cells were collected using CD45- and CD31+ immunomagnetic beads. (A) mRNA expression of ICAM-1 in PMVECs was increased significantly in the LPS group. The values presented are mean ± SEM. (n=15 in each group). Comparisons were made by one-way ANOVA, *P<0.05. (B) Immunohistochemistry (200×) revealed that ICAM-1 protein in LPS-stimulated mouse lung tissues was higher than in the control group or the LPS plus MMI-0100 group. (C) ICAM-1 protein level in mouse PMVECs was measured by Western blotting (left); quantitative densitometry of ICAM-1 protein level in mouse PMVECs (right). The values presented are the mean ± SEM. The results are representative of three independent experiments (*P<0.05).

Abbreviations: LPS, lipopolysaccharides; PMVECs, pulmonary microvascular endothelial cells.