Figure 5.

AQTGTGKT peptide, but not mutant peptides, binds to CAGE and regulates autophagc flux and the binding of CAGE to Beclin1. (A) PC-9/ER cells were transfected with FITC-AQTGTGKT peptide (10 μM) or unlabeled AQTGTGKT peptide (10 μM). At each time point after transfection, fluorescence microscopic observation was performed. Scale bar represents 10 μm. (B) PC-9/ER cells were transfected with the indicated peptide (each at 10 μM). At 24 h after transfection, immunoblot, and immunoprecipitation were performed. (C) PC-9/ER cells were transfected with the indicated peptide (each at 10 μM). At 24 h after transfection, immunoprecipitation was performed. (D) Same as (C) except that migration and invasion assays were performed. ^*p < 0.05; ^**p < 0.005; ^***p < 0.0005.