Figure 8.

AQTGTGKT peptide decreases tumorigenic potential of PC-9/ER cells. (A) PC-9/ER (1 × 10⁶) cells were injected into dorsal flanks of athymic nude mice. Following the establishment of sizeable tumor (~50 mm³), AQTGTGKT peptide (50, 100, 200 μg/mouse) was injected into the tail vein five times in a total of 41 days. Each experimental group consisted of five mice. Each value represents an average obtained from the five athymic nude mice of each group. Data are expressed as a mean ± S.D. Each figure shows a representative image of mouse in each group at the time of sacrifice. ^*p < 0.05; ^**p < 0.005. (B) Lysates from the indicated tumor tissues were subjected to immunoblot and immunoprecipitation employing the indicated antibody (2 μg/ml). (C) Immunohistochemical staining of tumor tissues employing the indicated antibody (2 μg/ml) was performed. Scale bar represents 100 μm.