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. Author manuscript; available in PMC: 2019 May 18.
Published in final edited form as: ACS Synth Biol. 2018 May 3;7(5):1179–1187. doi: 10.1021/acssynbio.7b00334

Figure 3.

Figure 3.

Secreted fatty acid and protein levels of E. coli expressing A. baylyi TE (AbTE) and AbTE mutants. A. Secreted fatty acid levels of E .coli expressing AbTE single, double, and triple arginine mutants, as well as AbTE single and double glutamate mutants. All experiments were done in triplicate and the error bars represent the standard deviation from the mean. B. Time course of saturated fatty acid titers produced by E. coli expressing AbTE, AbTE:G17R, and AbTE:G17R/A165R. C. Coomassie stained SDS-PAGE gel of Serial dilution of induced E. coli cultures expressing AbTE:WT, AbTE:G17R and AbTE:G17R/A165R.