Skip to main content
. 2018 Dec 9;24:8899–8909. doi: 10.12659/MSM.908628

Figure 4.

Figure 4

Relationship among Lin28A, c-Myc, and let-7a in BCPAP cells. (A) qPCR analysis was used to detect the mRNA expression of Lin28A, c-Myc, and let-7a in BCPAP cells transfected with Lin28A-siRNAs. The knockdown of Lin28A was associated with lower c-Myc and higher let-7a expression. (B) Western blot analysis was used to detect the protein expression of Lin28A and c-Myc expression in BCPAP cells transfected with Lin28A-siRNAs. The knockdown of Lin28A was associated with lower c-Myc levels. (C) qPCR analysis was used to detect the mRNA expression of let-7a, Lin28A, and c-Myc in BCPAP cells transfected with let-7a mimics. The overexpression of let-7a was associated with a decrease in Lin28A and c-Myc mRNA expression. (D) Western blot analysis was used to detect Lin28A and c-Myc protein expression in BCPAP cells transfected with let-7a mimics. The expression of Lin28A and c-Myc was downregulated by the overexpression of let-7a. Data are expressed as means ±SD of 3 independent experiments. * P<0.05; ** P<0.01; *** P<0.001, compared with media-only group (un).