FIGURE 7.

Distribution of NeuN- and GAD-immunopositive neurones in the model of pre-motor PD. (A) Distribution of GAD-67-immunopositive interneurons in the PC of the pre-motor model. (Aa) Example of GAD-67-immunostaining in the anterior PC of sham animals, model and model + EX-4 treatment at 19 days after surgery. (Ab) Cell densities in the PC at 19 days after surgery expressed as the number of GAD-67-positive neurones per mm³. The distributions were similar in all experimental groups. (B) Distribution of NeuN-immunopositive interneurons in the PC of the pre-motor model. (Ba) Example of NeuN-immunostaining in the anterior PC of sham animals and models with and without EX-4 treatment at 19 days after surgery. (Bb) Cell densities in the PC at 19 days after surgery are expressed as the number of NeuN-positive neurones per mm³ ± SEM. The density of NeuN-positive neurones in the pre-motor model increased compared with that in the sham animals. This increase was prevented by treatment with EX-4. ^∗∗∗∗P < 0.0001.