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. Author manuscript; available in PMC: 2018 Dec 17.
Published in final edited form as: Biochemistry. 2008 Dec 23;47(51):13659–13665. doi: 10.1021/bi801141q

Figure 2:

Figure 2:

Evaluation of mutants. (A) Sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE) analysis of protein samples used in kinetic trials. Ten microliters of the 10 μM sample was loaded for each mutant. (B) Cleavage tests of mutants. One micromolar enzyme samples were reacted with substrate, and aliquots were removed at 2 and 20 min time points for all samples.