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. 2018 Dec 17;13(12):e0206461. doi: 10.1371/journal.pone.0206461

Fig 1. Experimental design in our renal ischemic-reperfusion model.

Fig 1

(a) PAI was performed at different time points (before injury and at 4, 8, 12, 24 and 48 h after ischemia-reperfusion injury). The time period of renal ischemia-reperfusion was 35 min to induce mild AKI (14 mice) and 50 min to induce severe AKI (13 mice). Renal function, histological pathology and volume were measured 28 days after AKI induction. (b) L-NAME was administered from 24 h before ischemia-reperfusion to 24 h after reperfusion. PAI was performed at different time points (before injury and at 12 and 24 h after ischemia-reperfusion injury). The period of renal ischemia-reperfusion was 35 min to induce mild AKI (10 mice) and 50 min to induce severe AKI (10 mice).