Skip to main content
. 2018 Dec 17;13(12):e0208756. doi: 10.1371/journal.pone.0208756

Fig 2. RARRES1 regulates DNL.

Fig 2

(A) Citrate levels in stable RARRES1 knockdown MCF10A cells were calculated using MS/MS method and compared to empty vector MCF10A cells. (B) Glycolytic activity was assessed in MCF 10A and PWR-1E cells. The glycolytic reserve was calculated for both cell lines. (C) Transient RARRES1 KD and scramble control were treated with C75 inhibitor and glycolytic activity was assessed. Glycolytic usage and reserve were assessed in vehicle and C75 treated groups. (D) Scrambled siRNA or RARRES1 siRNA transfected cells were treated with vehicle or 40 μM C75 for 2 hours or 4 hours. Cells were then stained with Oil Red O and DAPI. (E) A schematic diagram of glycolysis and glucose dependent de novo lipogenesis pathway in RARES1 depleted epithelial cells and the effect of C75 on these cells is depicted. G: Glucose; O: Oligomycin; 2-DG: 2-Deoxy-D-glucose.