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. 2018 Dec 17;13(12):e0208756. doi: 10.1371/journal.pone.0208756

Fig 4. Differential expression of RARRES1 in cancers correlates with expression of fatty acid metabolism genes.

Fig 4

Cancers where RARRES1 gene was differentially expressed were chosen and correlative analysis was done. FASN, SCD, PPARG, SREBF1, PPARGC1A and PPARA, were chosen as candidate genes important in fatty acid oxidation and lipogenesis. (A) Three subtypes of breast cancer, 5 types of colorectal cancer and metastatic versus primary sites of prostate cancer were analyzed and fold change difference (with p-value <0.01) was plotted for each gene. (B) The correlative score (calculated using Pearson correlation formula) was calculated between two genes in all cancers analyzed. (C) Alggen PROMO software predicted PPAR alpha and gamma in complex with RXR to bind to non-coding regions of RARRES1 gene (S2 Table). (D) Endogenous PPAR agonist, DHA, regulates RARRES1 expression in MCF 10A cells. (E) Synthetic agonists of PPAR alpha (Fenofibrate) and gamma (Rosiglitazone and Pioglitazone) were used to treat MCF10A cells or primary hepatocytes for 48 hours.