. 2018 Dec 17;13(12):e0202238. doi: 10.1371/journal.pone.0202238

Table 1. Comparison of protein yield and spot number in 2DE between the two methods.

Maize tissues	The modified method		The classical method
Maize tissues	Yield ^a	Spot No ^b	Yield ^a	Spot No ^b
Roots	4.82±0.07	738±11	4.70±0.08	743±10
Leaves	4.13±0.11	310±9	4.06±0.23	314±4
Embryos	5.80±0.13	314±12	5.56±0.38	304±18

Notes

a, Protein yields are expressed as μg/mg fresh weight.

b, Average spot number detected in 2D gels using PDQUEST software (version 8.0, Bio-Rad).

All data were from at least three independent experiments. The corresponding data from the two methods did not differ significantly (p<0.05) according to t-test.