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Identification of IgY by Western blot. Lane 1 = BLUeye Prestained Protein Ladder, lane 2–7 = purified IgY, lane 8 = PiNK Plus Prestained Protein Ladder. Result of Western blot test using Anti-chicken IgY (IgG) (whole molecule) alkaline phosphatase antibody produced in rabbit to detect the heavy chains and the light chains of chicken IgY. The LC: light chain ≈ 65 kDa, the HC: heavy chain ≈ 27 kDa.
