Table 1.

Purification scheme of DNase from small intestine of camel.

Purification step	Total protein (mg)	Total unitsa	Specific activity (unit/mg protein)	Fold purification	% Recovery
Crude extract	3.15	3375	1071	1.0	100
Chromatography on DEAE-Sepharose
0.0 M NaCl (DNase 1)	0.78	1300	1666	1.55	38.5
0.1 M NaCl (DNase 2)	0.336	833	2479	2.31	24.6
0.2 M NaCl (DNase 3)	0.15	670	4466	4.16	19.8
Gel filtration on Sephacryl S-200
(DNase 3)	0.06	525	8750	8.16	15.5

^aOne unit of DNase activity is defined as the amount of enzyme that increases the optical density 1.0 per min under standard assay conditions.