Table 1.
S. No. | Primers | Sequence | GC% | Annealing temperature | Length |
---|---|---|---|---|---|
1. | F-1 Primer | CTGGACACAC | 60 | 45 °C throughout the experiment | 10 bp |
2. | OPC-06 | GAACGGACTC | 60 | ||
3. | OPC-07 | GTCCCGACGA | 70 | ||
4. | OPD-08 | GTGTGCCCCA | 70 | ||
5. | OPA-02 | TGCCGAGCTG | 70 | ||
6. | OPD-02 | GGACCCAACC | 70 | ||
7 | OPB-10 | CTGCTGGGAC | 70 |