Table 2.
Primer sequence, total number of bands (TB), number of polymorphic bands (PB), percentage of polymorphic bands (PB%), polymorphic information content (PIC) and marker index (MI), for ISSR, ITS-SSCP primers and ITS-RFLP restriction enzymes.
| Marker system | Primer | Primers sequence (5́–3́) | TB | PB | PB% | PIC | EMR | MI | |
|---|---|---|---|---|---|---|---|---|---|
| ISSR | ISSR3 | CACCACCACGC | 14 | 13 | 92.85 | 0.486 | 7.580 | 3.63 | |
| ISSR4 | GTGTGTGTGTGTCG | 10 | 9 | 90.00 | 0.339 | 6.260 | 2.123 | ||
| ISSR6 | GAGAGAGAGAGACC | 18 | 15 | 83.33 | 0.431 | 9.370 | 4.39 | ||
| Mean | 14 | 12.33 | 88.72 | 0.431 | 7.736 | 3.381 | |||
| ITS-RPLP | ITS 1 ITS4 |
EcoRV | 5́…GAT▾ATC..0.3́ 3́…CTA▲TAG..0.5́ |
4 | 4 | 100 | 0.395 | 2.166 | 0.472 |
| AluI | 5́…AG▾CT..0.3́ 3́…TC▲GA..0.5́ |
4 | 4 | 100 | 0.444 | 1.080 | 0.426 | ||
| HaeIII | 5́…GG▾CC..0.3́ 3́…CC▲GG..0.5́ |
1 | 1 | 0 | - | - | - | ||
| Mean | 4 | 4 | 100 | 0.419 | 1.623 | 0.449 | |||
| ITS-SSCP | ITS 1 ITS4 |
TCCGTA GGTGAACCTTGCGG TCCTCC GCTTATTGATATGC |
4 | 4 | 100 | 0.444 | 1.333 | 0.592 | |
| SSR | Pe17 | F: AAAATCATTTCAGGGTGC R: TACTTTGAGCCAGGTGCC |
5 | 4 | 80 | 0.480 | 2.400 | 1.152 | |
| Pe39 | F: TAGCAGAATCACAGAGTT R: CTAGAAATCCCACCAAAA |
4 | 2 | 50 | 0.186 | 3.550 | 0.666 | ||
| Pe145 | F: AATGGGGACGTGTTGTTA R: TGCAGATGGACGATGTTT |
5 | 1 | 20 | 0.095 | 2.163 | 0.090 | ||
| Pe217 | F: AAAAGCAGAACGCTCCCC R: CGGTGCCACGAAATAGTA |
4 | 2 | 50 | 0.218 | 0.950 | 0.382 | ||
| Mean | 4.50 | 2.25 | 0.244 | 2.163 | 0.572 | ||||