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. 2017 Jul 4;15(2):345–358. doi: 10.1016/j.jgeb.2017.06.012

Table 1.

Properties of RAPD primers used for polymorphic analysis of the wild and mutant strain of A. pullulans NAC8.

S/N Primer Primer sequence %GC content Melting temperature (Tm) Molecular weight
1 OPB05 5′-TGCGCCCTTC-3′ 70 34 3035.26
2 OPB08 5′-CCACAGCAGT-3′ 60 32 3077.33
3 OPH01 5′-GGTCGGACAA -3′ 60 32 3157.39
4 OPH02 5′-TCGGAGGTGA-3′ 60 32 3188.39
5 OPH06 5′-ACGCATCGCA-3′ 60 32 3077.33
6 OPT02 5′-GGAGAGACTA-3′ 60 32 3157.39
7 OPT04 5′-CACAGAGGGA -3′ 60 32 3206.43
8 OPT05 5′-GGGTTTGGCA-3′ 60 32 3179.35
9 OPT09 5′-CACCCCTGAG -3′ 70 34 3053.30
10 OPT19 5′-GTCCGTATGG-3′ 60 32 3139.35