Table 5b.

Analysis of Variance (ANOVA) for the Box Behnken model quadratic model for both extracellular and intracellular fructosyltransferase by the improved mutant strain.

Source	Sum of squares	DF	Mean square	F-value	Prob > F
Model	A1.98 × 106	9	2.2 × 105	32.110	<0.0001	Significant
	B1.75 × 105	9	19279.56	35.370	<0.0001	Significant
A: Sucrose	A1.29 × 105	1	11.29 × 105	18.740	0.0034	Significant
	B13240.37	1	13240.37	24.290	0.0017	Significant
B: NH4Cl	A1297.49	1	1297.49	0.190	0.6770	Not significant
	B17.72	1	17.72	0.033	0.8620	Not significant
C: Yeast extract	A15894.29	1	15894.29	2.320	0.1780	Not significant
	B868.38	1	868.38	1.59	0.2473	Not significant
Sucrose * sucrose	A2.146 × 105	1	2.146 × 105	31.28	0.0008	Significant
	B50166.80	1	50166.80	38.34	0.0004	Significant
NH4Cl * NH4Cl	A5.564 × 105	1	5.564 × 105	81.11	<0.0001	Significant
	B50166.80	1	50166.80	92.03	<0.0001	Significant
Yeast extract * yeast extract	A7.335 × 105	1	A7.335 × 105	107.21	<0.0001	Significant
	B61708.60	1	61708.60	113.21	<0.0001	Significant
Sucrose * NH4Cl	A48.25	1	48.25	7.03 × 10−3	0.9355	Not significant
	B3.94	1	3.94	7.23 × 10−3	0.9346	Not significant
Sucrose * yeast extract	A1.623 × 105	1	1.623 × 105	23.66	0.0018	Significant
	B11913.19	1	11913.19	21.86	0.0023	Significant
NH4Cl * yeast extract	A4509.06	1	4509.06	0.66	0.4442	Not significant
	B63.01	1	63.01	0.12	0.7438	Not significant
Residual	A48023.53	7	6860.50	–	–	–
	B3815.64	7	545.09	–	–	–
Lack of fit	A37857.84	3	12619.28	4.97	0.0778	Not significant
	B3249.05	3	1083.02	7.65	0.0392	Not significant
Pure error	A10165.69	4	2541.42
	B566.59	4	141.65
Correlation total	A2.031 × 106	16
	B1.773 × 105	16

R² for A is 0.98 while R² for B is 0.97;  CV for A is 2.53% while CV for B is 2.40.

Keys:

^AExtracellular FTase.

^BIntracellular FTase.