Table 2. Characteristics of anti-CHIKV mAbs.
| Strategy | mAb | Isotype H, L |
Western blot analysisa | Binding region |
|
|---|---|---|---|---|---|
| reducing | non-reducing | ||||
| B | 3D11 | IgG2a, κ | non-reactive | non-reactive | E1 |
| B | 4E3 | IgG1, κ | non-reactive | non-reactive | ND |
| B | 6B6 | IgA, κ | ND | ND | ND |
| B | 9E3 | IgG1, κ | non-reactive | non-reactive | ND |
| B | 11E11 | IgG1, κ | non-reactive | 48 kDa | E1 |
| B | 13H11 | IgG1, κ | non-reactive | non-reactive | E1 |
| B | 15B2 | IgG2b, κ | non-specific | non-specific | E1 |
| B | 19B8 | IgG2a, κ | non-reactive | non-reactive | E1 |
| B | RC5-3 | IgG1, κ | non-reactive | non-reactive | E1 |
| C | 24B3 | IgG1, κ | 35 kDa | 35 kDa | Capsid |
| C | 26A2 | IgG1, κ | 35 kDa | 35 kDa | Capsid |
| C | 32A3 | IgG1, κ | 35 kDa | 35 kDa | Capsid |
| C | 35B9 | IgM, κ | ND | ND | ND |
| C | 37C7 | IgG1, κ | 35 kDa | 35 kDa | Capsid |
| C | 41G5 | IgG1, κ | 35 kDa | 35 kDa | Capsid |
|
Control mAb |
CK47 | IgG2a, ND | 50 kDa | 48 kDa | E1 |
Note; H: Heavy chain; L: Light chain, ND: Not determined.
a Monoclonal antibody-containing culture supernatant versus CHIKV-infected cell lysate.