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. 2018 Dec 12;10:6857–6867. doi: 10.2147/CMAR.S188364

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© 2018 Jiang et al. This work is published and licensed by Dove Medical Press Limited

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HJC0152 induces apoptosis in GC cells

Note: (A) AGS (upper) and MKN45 (lower) cells were treated with HJC0152 (0, 5, 10, and 20 µM) for 24 hours, and stained with Annexin-FITC/PI. (B) Histograms showing the percentage of apoptotic cells in AGS (left) and MKN45 (right) cells. Data were presented as the mean ± SD of three independent experiments (C) Western blots showing the levels of apoptosis-related proteins in AGS and MKN45 cells treated with 20 µM HJC0152 for varying time durations (0, 1, 2, and 4 hours) or treated with different concentrations of HJC0152 (0, 5, 10, and 20 µM) for 4 hours. GAPDH served as the internal control. Data were collected from three independent experiments. **P<0.01, ***P<0.001 vs control.

Abbreviations: FITC, fluorescein isothiocyanate; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; GC, gastric cancer; PI, propidium iodide.