Figure 6.

(A) Validation of capture antibody conjugated with MBs by magneto-ELISA (color reaction): color reaction in tube 2 (ESAT-6 coated + MBs coupled with capture antibody) indicates the conjugation of capture antibody with MB, but no color reaction was observed in controls: 1) tube 1: ESAT-6 coated + MBs with no capture antibody; 2) tube 3: no ESAT-6 coated + MBs with no capture antibody; and 3) tube 4: no ESAT-6 coated + MBs with capture antibody. (B) Bar diagram revealed that OD* observed with MBs coupled with capture antibody was significantly higher (P<0.001) than the respective control, ie, MBs without capture antibody^#. All the controls revealed negligible OD values, thus validating the conjugation of capture antibodies with MBs.

Abbreviations: ESAT-6, early secreted antigenic target-6; MBs, magnetic beads; OD, optical density.