Fig 3. Enhancement of in vitro engulfment of patient-derived pancreatic ductal adenocarcinoma (PDAC) cells with CD47 blockade.

A) Schematic of the in vitro engulfment assay protocol. B) Percent engulfment of five patient-derived PDAC cell lines in the presence of purified IgG from mouse serum (IgG) or a blocking anti-CD47 antibody (αCD47). Bars represent mean percent engulfment under each condition, closed circles represent individual experiments, and the dashed lines connect data within an individual experiment. Experiments were performed twice, each with three technical replicates. Paired t-test was used to compare engulfment percentages under different treatments. Significance is denoted as * p<0.05 and ** p<0.01. C) Pearson’s correlation between CD47 receptor density index (RDI) and Δ Engulfment (Percent Engulfment_{αCD47-Treated}/Percent Engulfment_{Mouse IgG-Treated}).