Figure 4.

Fabrication of hydrogels with transient presentation of NCad mimetic peptides by metalloprotease ADAM10. (a) Schematic of MSCs within hydrogels containing transient peptides regulated by ADAM10, and transient NCad mimetic HAV peptide and control scrambled peptide sequences. (b) Release of FITC-labeled transient peptide coupled to the MeHA macromer from bulk hydrogels by ADAM10 and compared to control buffer without ADAM10. (c) Elastic modulus (E) and (d) mass swelling ratio of MeHA hydrogels without (grey) or with (orange) transient HAV peptide incorporated at 2 mM. (n ≥ 3 hydrogels per group, error bars represent s.e.m.); n.s. denotes no significant differences between groups.