Author response image 1. Overexpression of RNH1 or SEN1 does not suppress the sensitivity of Δsae2 strains to hydroxyurea (HU) exposure.

(A). RNH1 was induced from the GAL1 promoter on a CEN plasmid in Δsae2 compared to Δsae2 with vector only. Viability with or without HU (100 mM) was assessed at 48 hours or 72 hours as indicated. All plates contain galactose. (B). SEN1 was overexpressed from a CEN plasmid inΔsae2 compared to Δsae2 with vector only. Viability with or without CPT (5 μg/ml) was assessed at 48 or 72 hours as indicated on glucose-containing media.