Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (yeast) |
yeast strains see supplementary file 1 | |||
| Cell line (human) | U2OS | ATCC | ||
| Cell line (human) | HEK-293T | ATCC | ||
| Cell line (human) | U2OS T-RexTM Flp-in | Jeff Parvin | U2OS Flp-in | Invitrogen tet-on system |
| Transfected construct (human) |
pTP3146 | this study | GFP-CtIP | see Materials and methods |
| Transfected construct (human) | pTP3663 | this study | Flag-Bio-CtIP | see Materials and methods |
| Transfected construct (human) | pTP3665 | this study | Flag-Bio-CtIP(N289A/H290A) | see Materials and methods |
| Transfected construct (human) | pTP3531 | this study | C-term of Senataxin in pcDNA5/FRT/TO | see Materials and methods |
| Transfected construct (human) | pTP4195 | this study | PCF11 in pcDNA5/FRT/TO | see Materials and methods |
| Transfected construct (E. Coli) | pTP3492 | this study | wild-type RNaseH-mCherry in pcDNA5/FRT/TO | see Materials and methods |
| Transfected construct | pTP3494 | this study | mCherry only in pcDNA5/FRT/TO | see Materials and methods |
| Transfected construct (E. Coli) | pTP3493 | this study | RNaseHI-D10R-E48R in pcDNA5/FRT/TO | see Materials and methods |
| Transfected construct (E. Coli) | pTP3660 | this study | RNaseHI-D10R- E48R in lentivirus vector |
see Materials and methods |
| Transfected construct | pRSITEP--U6Tet-(sh)-EF1-TetRep-2A-shRNA-CtIP | Cellecta | inducible shRNA for CtIP | see Materials and methods |
| Transfected construct | pTP3703 | this study | inducible shRNA for XPG | see Materials and methods |
| Transfected construct | pTP3677 | this study | inducible shRNA for SETX | see Materials and methods |
| Transfected construct | pTP4195 | this study | Sγ3 sequence in pcDNA5/FRT/TO | see Materials and methods |
| Other | primers see supplementary file 4 |