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. Author manuscript; available in PMC: 2020 Jan 1.

Published in final edited form as: Acta Biomater. 2018 Oct 26;83:96–108. doi: 10.1016/j.actbio.2018.10.038

Figure 8. — MSC retention in thigh muscles of C57BL/6 mice. MSCs were labeled by live cell tracker CMDil. (A) Live MSCs in muscle tissues after 24 h of implantation. PNHAV15HYP, collagen and PBS were used as cell carriers; (B) quantification of MSC density in tissues based on the images. Scale bar = 100 μm. *p<0.01; Hydrogel fluorescent signal change and MSC survival after injection into thigh muscles of nude mice for 4 weeks. fluorescent images of hydrogel PNHAV15HYP during the 4‐week implantation period; and (D) confocal image of hydrogel and live MSCs (CMDil‐labeled) after 4 weeks of implantation. Scale bar = 100 μm. In A and D, the color for red fluorescent CMDil was changed to blue in order to clearly distinguish the CMDil‐labeled cells and hydrogel.

Figure 8. — MSC retention in thigh muscles of C57BL/6 mice. MSCs were labeled by live cell tracker CMDil. (A) Live MSCs in muscle tissues after 24 h of implantation. PNHAV15HYP, collagen and PBS were used as cell carriers; (B) quantification of MSC density in tissues based on the images. Scale bar = 100 μm. *p<0.01; Hydrogel fluorescent signal change and MSC survival after injection into thigh muscles of nude mice for 4 weeks. fluorescent images of hydrogel PNHAV15HYP during the 4‐week implantation period; and (D) confocal image of hydrogel and live MSCs (CMDil‐labeled) after 4 weeks of implantation. Scale bar = 100 μm. In A and D, the color for red fluorescent CMDil was changed to blue in order to clearly distinguish the CMDil‐labeled cells and hydrogel.