Figure 4.

OGT promotes antiviral immune responses in human cells. (A to F) Immunoblotting of phosphorylated TBK1, IKKε, IRF3 and STAT1 (A and D), and NF-κB (B and E) signaling molecules in OGT-KO and WT control THP-1 (A to C) or HT29 (D to F) cells challenged with VSV (MOI = 1) for indicated periods. Transcripts of IFNB1, IL6 and TNFA in the cells were measured with RT-PCR (C and F). (G and H) Protein O-GlcNAcylation (G) and cytokine transcripts (H) in OGT-KO THP-1 cells virally transduced with either empty vector or expression vector for human OGT with synonymous mutation that was not recognized by OGT gRNA, followed by VSV challenge for 6 h. * P < 0.05, versus controls (two-tailed Student’s t-test (C, F and H)). Data are from one experiment representative of three experiments (A, B, D, E and G) or represent four independent experiments (C, F and H; mean ± s.d. of four biological replicates).