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. 2018 Nov 8;11(1):99–108. doi: 10.1159/000494070

Fig. 1.

Fig. 1

LPS treatment altered macrophage differentiation. a Freshly isolated bone marrow precursors expressed the macrophage markers CSF1R, F4/80, and CD11b after 3-day culture in L929-conditioned medium. b Adding LPS to the medium (250 ng/mL for 16 h) on day 0 had no effect on CSF1R or F4/80 expression. However, treating bone marrow precursors with LPS after 2 (middle) or 7 (right) days decreased CSF1R (top panels) and increased F4/80 expression (bottom panels). Representative FACS data are shown. c MFI data from multiple 2-day experiments show that LPS reduced CSF1R (** p < 0.005, n = 11) and increased F4/80 (** p < 0.005, n = 11). d Each separate bone marrow isolette displayed similar trends in F4/80 and CSF1R expression following LPS treatment on day 2. e LPS increased mRNA expression of Adgre1 (the gene encoding F4/80) and the scavenger receptor Msr1 (CD204) (*** p < 0.001, n = 10).