Fig. 3.

Effect of MAPK inhibition (inh.) on LPS-induced changes in BMDM differentiation. a FACS of BMDM treated with LPS or LPS following 1-h pretreatment with a MAPK inhibitor cocktail. MAPK inhibition prevented the LPS-induced reduction in CSF1R expression. However, F4/80 expression was not affected (* p < 0.05, *** p < 0.001, n = 5). b, c Representative FACS experiment showing effects of LPS ± MAPK inhibition on F4/80 and CSF1R expression in BMDM. MAPK inhibition prevented the LPS-induced changes in the percentage of F4/80+ cells also expressing CSF1R. Each line in (c) represents an independent experiment.