Fig. 4.

LPS caused persistent changes in BMDM differentiation and response to TLR agonists. a The LPS-induced changes in CSF-1R and F4/80 expression were unchanged after LPS was removed for 24 h. b LPS-treated BMDM (for 16 h on day 2) were challenged with a second treatment of either LPS again or the TLR2 agonist Pam₃CysSerLys₄ (PAM3) on day 4. c LPS treatment on day 2 increased the relative expression of Il6 following PAM3 stimulation on day 4 (* p < 0.05, n = 6). d Release of IL-6 and IL-10 into the cell culture medium after either LPS or PAM3 exposure on day 4 was not affected by LPS treatment on day 2.