Fig. 5.

LPS treatment during BMDM differentiation reduced phagocytosis. BMDM treated on day 2 or day 7 with LPS (250 ng/mL for 16 h) were then cultured with pHrodo-labeled E. coli BioParticles. Phagocytosis (endocytosis and intravesicular acidification) was measured by FACS. a, b Representative FACS plot (of 9 total experiments) showing populations of low fluorescence (PE^LO) and high fluorescence (PE^HI) within BMDM cultures. c, d Percentage of cells with low (PE^LO) or high (PE^HI) pHrodo fluorescence. Nine experiments were performed on cells from 3 separate bone marrow isolates. Data were normalized to controls (control = 100%). In the day 2 experiments (c), LPS increased the percentage of cells with low pHrodo E. coli fluorescence and decreased cells with a higher fluorescence intensity (**** p < 0.001). BMDM treated with LPS on day 7 (d) had similar percentages of cells with low or high pHrodo intensity compared to controls.