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. Author manuscript; available in PMC: 2019 Dec 1.
Published in final edited form as: FEBS J. 2018 Nov 20;285(24):4674–4683. doi: 10.1111/febs.14689

Fig. 2. The R3S12R3 peptide derived from chicken phosvitin 2 is readily phosphorylated by Fam20C, but not by CK1 and CK2.

Fig. 2.

A) Time course phosphorylation of R3S12R3 and β28–40 peptides in the presence of 200μM ATP. B) kinetics of R3S12R3 phosphorylation by Fam20C in the absence or presence of 25μM sphingosine. C) Stimulatory effect of Fam20A added to Fam20C in 20 fold molar excess, in the presence of 200 μM ATP. Incubation time: 60 min. D) R3S12R3 phosphorylation by Fam20C, CK2, and CK1 expressed as per count of the respective canonical peptides (β28–40, CK2 tide and CK1 tide respectively). Reported values are means ± SD of three independent experiments; for details see Materials and Methods.