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Protein immunoblotting of V5 immunoprecipitates from conditioned medium of U2-OS cells cotransfected with V5-tagged Danio rerio phosvitin (drpV:V5) and Fam20C WT or catalytic mutant D478A (DA). The total extracts were also analyzed for V5 by immunoblotting. Note that only the immunoprecipitates from conditioned medium were treated with phosphatase.
