TABLE 1.
Comparative Proteomic Analysis of Control Mice (NRV) and Irradiated Mice (RDV) Reveals Differentially Expressed Proteins
| Gene symbol | Description | Relative fold change (RDV/NRV) | P value | FDR adjusted P value |
|---|---|---|---|---|
| Upregulated | ||||
| Gucy1a2 | Guanylate cyclase 1, soluble, alpha 2 | 68.71 | 2.34E–08 | 3.58E–05 |
| Tmem205 | Transmembrane protein 205 | 54.70 | 3.94E–08 | 4.38E–05 |
| Gatm | Glycine amidinotransferase (l-arginine:glycine amidinotransferase) | 117.8 | 9.43E–07 | 9.62E–04 |
| Col5a2 | Collagen, type V, alpha 2 | 8.28 | 6.08E–06 | 3.10E–03 |
| Vwa5a | von Willebrand factor A domain containing 5A | 5.40 | 1.46E–05 | 7.15E–03 |
| Aldh3b2 | Aldehyde dehydrogenase 3 family, member B2 | 82.73 | 2.04E–05 | 9.61E–03 |
| Aldh3b1 | Aldehyde dehydrogenase 3 family, member B1 | 83.07 | 2.19E–05 | 8.13E–03 |
| Vcam1 | Vascular cell adhesion molecule 1 | 3.66 | 3.88E–05 | 1.16E–02 |
| Ctnna1 | Catenin (cadherin associated protein), alpha 1 | 5.01 | 7.09E–05 | 1.97E–02 |
| Hmgcs2 | 3-Hydroxy-3-methylglutaryl-coenzyme A synthase 2 | 98.45 | 7.55E–05 | 2.05E–02 |
| Pcca | Propionyl-coenzyme A carboxylase, alpha polypeptide | 94.82 | 9.75E–05 | 2.54E–02 |
| Ctnna2 | Catenin (cadherin associated protein), alpha2 | 86.78 | 1.33E–04 | 3.26E–02 |
| Anpep | Alanyl (membrane) aminopeptidase | 2.06 | 1.47E–04 | 3.28E–02 |
| Ctnnb1 | Catenin (cadherin associated protein), beta 1 | 4.41 | 1.55E–04 | 3.39E–02 |
| Lama5 | Laminin, alpha 5 | 2.17 | 2.15E–04 | 4.31E–02 |
| Ctnnd1 | Catenin (cadherin associated protein), delta 1 | 4.36 | 3.33E–04 | 4.38E–02 |
| Downregulated | ||||
| Gimap7 | GTPase, IMAP family member 7 | 0.016 | 1.29E–25 | 1.58E–21 |
| Ppp6r3 | Protein phosphatase 6, regulatory subunit 3 | 0.016 | 9.29E–25 | 5.68E–21 |
| Mcpt8 | Mast cell protease 8 | 0.015 | 1.20E–23 | 3.67E–20 |
| Grap2 | GRB2-related adaptor protein 2 | 0.016 | 2.49E–23 | 6.10E–20 |
| Pcsk4 | Proprotein convertase subtilisin/kexin type 4 | 0.034 | 7.31E–15 | 1.49E–11 |
| Coro1a | Coronin, actin-binding protein 1A | 0.011 | 1.67E–12 | 2.92E–09 |
| Sh3kbp1 | SH3-domain kinase binding protein 1 | 0.019 | 2.37E–06 | 1.52E–03 |
| Fam107b | Family with sequence similarity 107, member B | 0.023 | 2.15E–05 | 8.24E–03 |
| Add1 | Adducin 1 (alpha) | 0.114 | 3.28E–05 | 1.06E–02 |
| Nmral1 | NmrA-like family domain containing 1 | 0.083 | 3.75E–05 | 1.18E–02 |
| Frg1 | FSHD region gene 1 | 0.013 | 1.18E–04 | 2.95E–02 |
| Samp | SAP domain containing ribonucleoprotein | 0.017 | 1.47E–04 | 3.45E–02 |
| Polr2d | Polymerase (RNA) II (DNA directed) polypeptide D | 0.021 | 1.91E–04 | 4.02E–02 |
| Pml | Promyelocytic leukemia | 0.101 | 2.13E–04 | 4.34E–02 |
| Ctcf | CCCTC-binding factor | 0.100 | 2.72E–04 | 4.75E–02 |
| Lpxn | Leupaxin | 0.058 | 3.34E–04 | 4.34E–02 |
| Hmha1 | Histocompatibility (minor) HA-1 | 0.126 | 3.75E–04 | 4.83E–02 |
| Septin 1 | Septin 1 | 0.106 | 3.95E–04 | 4.98E–02 |
Notes. The protein intensity values were log2 transformed, centered by the median and scaled based on the standard deviation so that the data distribution had a median of 0 and a standard deviation of 1. The average intensity for each protein was used to calculate the relative fold change. Student’s t test was used to calculate the P values, and they were false discovery rate (FDR)-adjusted to account for multiple tests.