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. 2018 Dec 17;50(12):166. doi: 10.1038/s12276-018-0192-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a, b Flow cytometric analysis of systemic (circulation) and local (bone marrow) CD3⁺ T-cell populations. GNX, gonadectomy; MSCT, mesenchymal stem cell transplantation in GNX mice. (c, d) Corresponding quantification of total CD3⁺ T cells. PBMNCs peripheral blood mononuclear cells, BMMNCs bone marrow mononuclear cells. n = 3 per group. e, f Serological markers of inflammation. TNF-α tumor necrosis factor-alpha, IFN-γ interferon-gamma, n = 5 per group. g, h Flow cytometric analysis of the apoptotic rate of CD3⁺ T cells from GNX mice in co-culture with or without mesenchymal stem cells (MSCs). i, j Inflammatory cytokine concentrations in the co-culture media. n = 3 per group. The data represent the means ± SD. *P < 0.05; NS not significant (P > 0.05)