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. 2018 Dec 17;8:17907. doi: 10.1038/s41598-018-35849-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of centrifugation on erythrocyte shape change. Washed erythrocytes were sedimented at 1 g to 6,000 g to examine the influence of mechanical deformation on shape changes. (A) Scanning electron microscopy was used to visualize the centrifuged erythrocytes. Scale bar is 10 µm. Scanning electron microscopy images were quantified for the percentage of (B) biconcave (C) intermediate (mainly biconcave), and (D) intermediate (mainly polyhedral). Statistical analysis was completed using a one-way ANOVA with an alpha value of 0.5 where significant differences are shown with respect to 1 g (*), 100 g. Data is represented as a mean ± SEM. N > 100 cells for each experimental condition.