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. 2018 Dec 11;9:612. doi: 10.3389/fgene.2018.00612

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Copyright © 2018 Sollazzo, Genchi, Paglia, Di Giacomo, Pession, de Biase and Grifoni.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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MYC and PI3K^CAAX overexpression do not induce reciprocal activation. (A–A2) Immunostaining for pAKT (A,A1, cyan) and MYC (A2, red) on wing discs from late yw/yw, UAS-PI3K^CAAX; hh-Gal4, UAS-GFP/+ larvae. (B) Graph comparing the Mean Fluorescence Intensity Arbitrary Units (MFI-AU) of MYC staining measured in the P (green bar) and A (grey bar) compartments of 15 wing discs from different larvae. (C,C1) Immunostaining for pAKT (cyan) on wing discs from late yw; hh-Gal4, UAS-GFP/UAS-dm larvae. (D) Graph comparing the Mean Fluorescence Intensity Arbitrary Units (MFI-AU) of pAKT staining measured in the P (green bar) and A (grey bar) compartments of 15 wing discs from different larvae. Basic genotypes are indicated on the left of the figure panel. P compartments are outlined in A2,C1, and disc axes are indicated in A,C. Magnification is 400×.